Comparative bacteriological characteristics of wound regeneration processes using various suture materials in laboratory animals

Abstract

Reparative regeneration of skin wounds largely depends on the chemical composition, structure and quality of the adhesive or suture material as well as the reaction of tissues to its implantation. Microbial contamination of the wound is of particular importance in the process of tissue regeneration, because it may lead to various complications. Intensive development of microbial contamination in the wound not only slows down its healing but also creates a risk of surgical infection in the body. The interpretation of the results of a bacteriological study of the microbiota of surgical wounds sutured with background materials is of great practical importance for further study of adhesive compositions. The research goal was to study the qualitative and quantitative composition of skin wound microflora when background suture materials were used. It was found that representatives of the skin microflora of Staphylococcus epidermidis and a representative of the intestinal microflora Escherichia coli were present in the skin wounds of all laboratory animals; in addition, Acinetobacter species and Klebsiella pneumoniae were isolated from mice of the 2nd trial group. Microbiological control on the 3rd, 7th and 14th days showed the absence of microbiota in skin wounds in animals of the 1st trial group and decreasing qualitative and quantitative composition of microflora in skin wounds in mice of the 2nd trial group.