Effect of protein preparation from bone marrow cells on broiler chicken immunity during vaccination against chicken infectious bronchitis

Abstract

Infectious bronchitis of chickens causes huge economic damage to broiler poultry farming. The economic damage is mainly associated with the high mortality of broiler chickens under the age of 30 days. The main condition for ensuring epizootic well-being regarding chicken infectious bronchitis is the effectiveness of immunization. The research goal was to evaluate the effect of a protein preparation from bovine bone marrow cells on the immunity of vaccinated broiler chickens against the infectious bronchitis virus in chickens. By the end of the experiment, significant increase of total protein and gamma globulins in blood serum by 10.73% and 13.87% was recorded. The use of the preparation contributed to increased level of immunoglobulins by 5.42%. Lysozyme activity increased by 7.51% while bactericidal activity increased by 8.10%. The difference in the phagocytic activity of neutrophils compared to the control group was 4.04%, and the phagocytic index increased by 6.49%. The administration of the protein preparation from bone marrow cells to broiler chickens against the background of vaccination against IBV contributed to increased geometric mean values of antibody titers by 20.44%, titer arithmetic mean values - by 31.53%; log2 - by 0.20%; SD - by 48.60% as compared to the control. The coefficient of variation (CV) in the trial group was 39.2%. The minimum antibody titers in the trial group of broiler chickens were found in six (26.08%) samples (from 464 to 958), the mean titers - 16 (69.56%) samples (from 1367 to 3949), the maximum - in one (4.34%) sample (6378). The intensity of group immunity in the trial group was 100.00%. These indices show a sufficient and uniform level of specific body protection for broiler chickens. The use of the protein preparation from bone marrow cells in the schemes of specific prevention of chicken infectious bronchitis in broiler chickens will ensure epizootic well-being regarding this disease on poultry farms.