Effect of silver nanoparticles and Biosib®ATSID on Staphylococcus aureus biofilms

Abstract

Microbial biofilms are communities of aggregated microbial cells that are embedded in a self-produced matrix of extracellular polymeric substances. Biofilms are resistant to extreme environmental conditions and can protect microorganisms against ultraviolet radiation, extreme temperatures, extreme pH values, high salinity, high pressure, poor nutrients, antibiotics, etc., acting as “protective clothing”. The development of highly effective antibacterial agents for the destruction of biofilms and bacteria is of great importance. In recent years, biofilm research has mainly focused on biofilm-related infections and microbial biofilm control strategies. We have determined the effect of Argovit + Biosib®ATSID products on biofilm formation in Staphylococcus aureus isolated from cattle with a clinical manifestation of an infectious disease. The studies have shown that the use of a product based on silver nanoparticles and Biosib®ATSID significantly reduced the level of biofilm formation in both the reference strain and the Staphylococcus aureus isolate, and significantly increases the bactericidal properties of this composition. It is found that the use of enzyme products and antibacterial agents based on metal nanoparticles as complex preparations is an effective means of combating biofilms. The reduction of biofilm formation in Staphylococcus aureus ATCC 25953 was achieved to 23.32%, and in its isolate - to 9.2%, which may be explained by the fact that the isolate has high adaptive capabilities and high pathogenicity and virulence acquired during infection.