Determination of residual amounts of silver nanoparticles in milk, blood and urine in the treatment of mastitis and purulent-catarrhal endometritis in cows

Abstract

Currently, the leading place among pathologies in animals is occupied by multi-factor infectious diseases which etiology involves opportunistic pathogenic microflora. At the same time, it is known that the formation of pathological microbiocenoses in animals inevitably leads to associated epizootic processes associated with pathogens belonging to the category of opportunistic pathogens which under the conditions of intensive livestock farming may cause mass factor infections that cause significant damage to the industry. Factorial infectious diseases are epizootic in nature and may manifest themselves in the mass nature of gynecological diseases and mastitis in cows. In the past decade, there was increasing tendency of bovine mastitis and endometritis incidence. The main etiological factor of mastitis is the presence of a predisposing factor and various associations of opportunistic pathogenic microflora. The main methods of treatment of mastitis and endometritis are based on the use of drugs aimed at suppressing the vital activity of bacteria and eliminating signs of inflammation. Under certain conditions, the use of modern antibacterial and pathogenetic therapies may lead to the formation of antibiotic-resistant strains of microorganisms which inevitably reduces the effectiveness of therapeutic and preventive measures. Currently, there are effective nanotechnology solutions that open up wide prospects for the creation of new drugs. Silver nanoparticles are the most promising for creating effective antibacterial agents for a wide range of pathologies. The increased interest in the use of silver nanoparticles is due to its high antibacterial activity against most pathogens. The research findings show that after treatment of bovine mastitis by Argovit drug, 24 hours after intracisternal administration and 12 hours after intrauterine administration, milk contains a concentration of silver ions below the maximum permissible concentration.