Effect of combined anthelmintic drug Alivek on the smooth muscles of an isolated segment of the intestine

Abstract

The combined anthelmintic drug Alivek was tested on the smooth muscles of an isolated intestinal segment. AliveK was tested as an aqueous suspension at a dilution of 1:10000, 1:1000, 1:500, and 1:250. Altogether, 12 tests were conducted. Dilution of Alivek anthelmintic at a concentration of 1:10000 had no significant effect on the activity of isolated intestinal tracts. The first significant change in the motor activity of isolated intestinal tracts occurred under the influence of the drug suspension 1:1000. When increasing the concentration of the investigated drug (1:500; 1:250) at one hour exposure, practically the same effect as in the previous dilution was observed but it manifested itself in 6-9 min, and tonus decrease was more intense. Well-known pharmacologic tests with acetylcholine chloride and barium chloride were used. The exposure to Alivek (1:250) for 30 min reduced intestinal tonus and prevented rhythmic intestinal contractions as expected. Against this background, acetylcholine chloride (1:100000) induced characteristic changes during the first minute of exposure. At the same time, intestinal tonus increased strongly, contractions became arrhythmic, and their amplitude decreased 2-3 times due to incomplete relaxation of the musculature. Alivek does not possess cholinolytic actions and hypotensive actions on the part of the tested drug are not observed. This is due to the fact that the studied drug Alivek does not eliminate the acetylcholine effect. This series of experiments shows that the new anthelmintic compound Alivek at a dilution of 1:1000 has a hypotensive effect on the smooth muscle of the rabbit intestine changing the character of pendulous contractions and slightly reducing the amplitude of intestinal contractions.