Cellular and immunobiochemical indices of calf blood when using immunomodulators of animal origin

Abstract

The immunomodulators that affect the cellular and immunobiochemical indices of calf blood are studied. The ways and methods of solving problems using new immunomodulatory agents of activating the immunoprotective forces of the animal body are discussed. We used the following immunomodulatory agents: a globulin preparation developed by us from cow colostral whey (Globulinsorbin plus), and a peptide preparation from the wastes of pharmaceutical processing of raw materials of velvet antler deer (Pantolysate plus). A series of production experiments to study the effects of these agents on calf immunity were carried out on 90 animals of the same age on separate farms of the Amur Region free from infectious diseases. On each farm, three groups of 10 Holstein-Friesian calves (two trial groups and one control group) were formed. The animals of the first trial groups were given 5% solution of the globulin agent daily, and the animals of the second trial groups were given 5% solution of the peptide agent. The agents were given daily during the first nine days of the animal life three times a day with 500 mL of warm milk 30 minutes before feeding. The animals of the control groups were given physiological salt solution according to a similar scheme. Blood samples were taken from the jugular vein on the 9th, 18th and 30th days of calf life. The use of the agents Globulinsorbin plus and Pantolysate plus promotes increase of cellular and immunobiochemical indices of calf blood. This fact is confirmed in comparison with the control by the maximum increase of total blood protein in calves by 28.54% and 18.49% by 9 days’ age, respectively. On the 30th day, its slight decrease was observed. Drinking these agents contributed to increase of blood serum bactericidal activity by 38.21% and 29.20%, respectively, by the 30th day. Lysozyme activity of calf blood serum in response to the agent from colostral whey during the entire observation period changed slightly from 51.28% to 44.60%. In response to Pantolysate plus, lysozyme activity of blood serum was different: the maximum increase was observed on the 30th day - by 36.32%. Phagocytic activity increased in response to both agents on the 30th and amounted to 12.72% and 10.15%, respectively. The maximum increase of immunoglobulins in response to Gglobulinsorbin plus was observed on the 9th day - by 28.39% and insignificant decrease on the 30th day. The amount of immunoglobulins in blood serum increased on the 30th day by 17.25%. Thus, the agents used significantly affected the cellular and immunobiochemical indices of calf blood. More intense changes of blood serum indices in calves were found when using Globulinsorbin plus. This effect may prevent immunodeficiency state in animals with reduced immunoresistance.