Improving the technology of sterilization of raspberry explants and accelerating morphogenesis in vitro culture

Abstract

Various aspects of the development of raspberry explants at the stage of their introduction into in vitro culture were studied. The research goal was to find out which drugs and treatment procedures had the greatest impact on the effectiveness of sterilization and the further development of explants in vitro culture. In addition, the optimal composition of growth promoters for successful development of raspberry explants was studied. The obtained results showed that for the introduction in vitro culture of both ever-bearing varieties and varieties with common fruiting, an exposure of 10 minutes turned out to be optimal when treated with Domestos product in a concentration of 1:1 and Lysoformin with an exposure of 10 minutes. In all raspberry varieties, the experiment revealed that increasing treatment time of explants introduced into the culture led to tissue damage, cessation of growth and development, and a shorter exposure time, in turn, led to insufficient sterilization of the starting material, and as a result, the overgrowing of the nutrient medium with pathogenic microflora after time has elapsed. To accelerate the development of regenerants, it is necessary to add growth promoters to the nutrient medium at a concentration of 6-BAP 5.0 µM + IBA 0.5 µM + HA 1.5 µM. When grown on this medium, the regenerated plants had a greater number of leaves and the highest shoot height. It was revealed that the combined effect of sterilizers and various growth promoters on the development of raspberry plants made it possible to identify the Atlant variety among varieties with ever-bearing type of fruiting, and the Barnaulskaya variety among varieties with a common type of fruiting. When further improving the technology of microclonal propagation of raspberries, it is necessary to take into account the data obtained.