Use of lysozyme in bovine mastitis treatment

Abstract

The research goal is to use alternative means of bovine mastitis treatment. The research was carried out at the Department of Morphology, Surgery and Obstetrics of the Faculty of Veterinary Medicine of the Altai State Agricultural University and on the farm of the OA Uchkhoz “Prigorodnoye” in the City of Barnaul. Fifteen cows were selected with clinical signs of mastitis (complete or partial lesion of the udder in the form of increased lobes, udder compaction, soreness during palpation, enlargement of the supramammary lymph glands) confirmed by laboratory blood and milk tests using Mastest-AF and Cenotest diagnostics. Under the conditions of the Department, lysozyme was isolated from cow milk by the method of V.I. Mutovin and tested for toxicity (the study was conducted on albino white mice) and for therapeutic efficacy on cows. The animals were divided into three trial groups of 5 comparable cows. In the 1st trial group, Mastisan drug was injected (15.0 mL into the teat cistern of the affected part of the udder 1 time a day for 5 days). In the 2nd trial group, Vaccamastum drug was administered intramammary (10.0 mL per head per day for 4 days). In the 3rd trial group, the experimental drug of the lysozyme group was injected intramammary into the affected quarter of the udder in a dose of 0.5 g for the first administration, then 0.25 g once a day depending on the severity of the pathological process for 5 days. As a result of comparing the treatment regimens in the three trial groups of cows, it was revealed that the use of the drugs Mastisan, Vaccamastum and the experimental drug of the lysozyme group was effective in the treatment of bovine mastitis. A distinctive feature of lysozyme from other drugs is the use of milk after complete recovery of the animal.