Effect of silver nanoparticles and feed preservative on microbial biofilm formation in vitro and in vivo

Abstract

A biofilm is a conglomeration of microorganisms located on a surface with their cells attached to each other. A biofilm has a spatial and metabolic structure of a microbial community located on the interface between two media. The studies of the effect of Argocide product on microorganisms in vitro showed a decrease of biofilm formation in St. aureus ATCC 25953 by 63.8% (from 1.41 + 0.01 to 0.51 ± 0.01), St. epidermidis ATCC 14990 - by 56.9% (from 1.23 + 0.03 to 0.53 ± 0.01), Str. pyogenes ATCC 19615 - by 60.7% (from 1.12 + 0.02 to 0.44 ± 0.02), S. enteritidis 182 - by 62.1% (from 1.24 + 0.01 to 0.47 ± 0.01), Pr. vulgaris ATCC 6380 - by 56.4% (from 1.17 + 0.01 to 0.51 ± 0.02), P. aeruginosa ATCC 27853 - by 67.9% (1.17 + 0.01 to 0.42 ± 0.01), E. coli ATCC 25922 - by 69.8% (1.26 + 0.01 to 0.38 ± 0.01), K. pneumoniae 71 - by 62.3% (from 1.22 ± 0.06 to 0.46 ± 0.02). The decrease of this indicator for the Biosib Acid^® product was higher (39.3-56.7%) than for Argovit^® product (14.3-32.0%). When treating calves with Lexoflon®, an increase of biofilm formation rate of the reisolated microflora was observed. Thus, in S. enteritidis, the increase of the value made 25.5% (from 0.90 + 0.03 to 1.13 ± 0.06), Pr. vulgaris - 34.9% (from 0.83 + 0.01 to 1.12 ± 0.04), P. aeruginosa - 22.1% (from 0.95 + 0.02 to 1.16 ± 0.07), E. coli - 34.9% (from 0.99 + 0.01 to 1.2 ± 0.06), K. pneumoniae - 38.6% from 0.88 ± 0.05 to 1.22 ± 0.08. The use of a laboratory sample of the Argocid product in the treatment of calves significantly reduced the process of microbial biofilm formation unlike the use of Lexoflon^® which determined its therapeutic effect and indicated that there were no negative changes in the ecological characteristics of microorganisms.