Study of the local intolerance of a complex agent from plant raw materials by the method of cutaneous applications

Abstract

The research goal was to conduct preclinical tests and determine the local intolerance of a complex herbal remedy using the method of skin applications. Four trial groups of 10 guinea pigs were formed. On the sides of laboratory animals, 2 patches of skin 2 × 2 cm were sheared. In the 1st group, the ointment of a complex remedy from vegetable raw materials prepared on petrolate at a dose of 50 mg g was evenly applied to the right side, once a day, for 20 days. In the 2nd group, the ointment of a complex remedy from vegetable raw materials prepared on petrolate at a dose of 300 mg g was evenly applied once a day, for 20 days; in the 3rd group, the ointment of a complex remedy from vegetable raw materials prepared on petrolate was evenly applied at a dose of 3000 mg g, once a day, for 20 days; in the 4th group - at a dose of 4000 mg g, once a day, for 20 days. Petrolate was similarly applied to the left side in the 1st, 2nd, 3rd and 4th groups without a complex agent. The skin response was visually assessed after 30 minutes, 24 hours, 48 hours, 7 days, 14 days, 20 days and a week after the end of the study. The findings obtained indicate the absence of any allergic response the trimmed skin areas which indicates that the complex remedy from plant raw materials does not cause any local intolerance on the skin of guinea pigs and may be classified as hazard class IV (low-risk substances).