Investigation of acute toxicity of a complex product of plant raw material in rodents

Abstract

The search for bioactive compounds of natural origin, also called phytobiotics, has become a serious problem for the industry, farmers and scientists. Plant-based feed supplements are plant-based products used in animal nutrition to improve productivity. This class of feed supplements has recently attracted increasing interest, as evidenced by a significant increase in the number of scientific publications since 2022. The research goal was to conduct preclinical tests to determine the acute toxicity of a complex herbal product in rodents. Two groups of 20 animals were formed. The trial group received a phytobiotic product mixed in water at a dose of 5 mg kg of body weight with a daily dose increase to 50, 300, 2000 and 5000 mg kg for 5 days. The growth dynamics of white outbred mice, biochemical and morphological blood indices were studied. A macroscopic examination of internal organs as the liver, kidneys, and spleen was also performed. The results obtained indicate the stimulating effect of the product on the growth dynamics and the absence of a negative effect on the animal body. This phytobiotic product has a stimulating effect on erythropoiesis, hemoglobin biosynthesis and other blood indices which in all trial groups varied within the physiological range; this indicates the absence of a negative effect of the phytobiotic product on the animal body. When analyzing the biochemical blood indices of mice in the trial and control groups, no deviations from the physiological standard were detected, and no deaths of animals were observed throughout the experiment, which was the basis for establishing hazard class IV (low-hazard substances).